Gene expression analyses on multi-target mode of action of black cohosh in menopausal complaints - a pilot study in rodents.

ABSTRACT:

Purpose

This study aimed at assessing gene expression profiles in hippocampus and hypothalamus of ovariectomized (OVX) rats with or without treatment with an isopropanolic extract of Cimicifuga racemosa rhizomes (iCR) in comparison to intact rats.

Methods

Exploration of hippocampal (Hi) and hypothalamic (Hy) tissue from Sprague Dawley rats: without OVX (N_Hi = N_Hy = 4), tissues 3 months after OVX (N_Hi = 4, N_Hy = 3), or tissues of rats after their treatment with iCR for 3 months after OVX (N_Hi = N_Hy = 2). Gene expression profiles in these tissues were investigated by RNA-microarray-analysis and subsequent verification by qPCR.

Results

4812 genes were differentially regulated when comparing the three group

SUBMITTER: Stute P 

PROVIDER: S-EPMC8782806 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature