Dynamic activity of interpeduncular nucleus GABAergic neurons controls expression of nicotine withdrawal in male mice.

ABSTRACT: A critical brain area implicated in nicotine dependence is the interpeduncular nucleus (IPN) located in the ventral midbrain and consisting primarily of GABAergic neurons. Previous studies indicate that IPN GABAergic neurons contribute to expression of somatic symptoms of nicotine withdrawal; however, whether IPN neurons are dynamically regulated during withdrawal in vivo and how this may contribute to both somatic and affective withdrawal behavior is unknown. To bridge this gap in knowledge, we expressed GCaMP in IPN GABAergic neurons and used in vivo fiber photometry to record changes in fluorescence, as a proxy for neuronal activity, in male mice during nicotine withdrawal. Mecamylamine-precipitated withdrawal significantly increased activity of IPN GABAergic neurons in nicotine-depende

SUBMITTER: Klenowski PM 

PROVIDER: S-EPMC8782840 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature