Unique high Arctic methane metabolizing community revealed through in situ ¹³CH₄-DNA-SIP enrichment in concert with genome binning.

ABSTRACT: Greenhouse gas (GHG) emissions from Arctic permafrost soils create a positive feedback loop of climate warming and further GHG emissions. Active methane uptake in these soils can reduce the impact of GHG on future Arctic warming potential. Aerobic methane oxidizers are thought to be responsible for this apparent methane sink, though Arctic representatives of these organisms have resisted culturing efforts. Here, we first used in situ gas flux measurements and qPCR to identify relative methane sink hotspots at a high Arctic cytosol site, we then labeled the active microbiome in situ using DNA Stable Isotope Probing (SIP) with heavy ¹³CH₄ (at 100 ppm and 1000 ppm). This was followed by amplicon and metagenome sequencing to identify active organisms involved in CH_4<

SUBMITTER: Altshuler I 

PROVIDER: S-EPMC8782848 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature