Ontology highlight
ABSTRACT:
SUBMITTER: Yoon S
PROVIDER: S-EPMC8783203 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
STAR protocols 20220120 1
This protocol describes using fluorescence recovery after photobleaching (FRAP) of a superecliptic pHluorin (SEP)-diacylglycerol lipase α (DAGLα) to measure membrane-bound DAGLα mobility in dendritic shafts of primary cultured cortical mouse neurons. This could serve as an excellent tool to analyze endocannabinoid-mediated synaptic plasticity. We have used this protocol to show that DAGLα surface dynamics play an integral role in regulating the dendritic spine. We also detail how we test the qua ...[more]