An overlooked subset of Cx3cr1^wt/wt microglia in the Cx3cr1^{CreER-Eyfp/wt} mouse has a repopulation advantage over Cx3cr1^{CreER-Eyfp/wt} microglia following microglial depletion.

ABSTRACT:

Background

Fluorescent reporter labeling and promoter-driven Cre-recombinant technologies have facilitated cellular investigations of physiological and pathological processes, including the widespread use of the Cx3cr1^{CreER-Eyfp/wt} mouse strain for studies of microglia.

Methods

Immunohistochemistry, Flow Cytometry, RNA sequencing and whole-genome sequencing were used to identify the subpopulation of microglia in Cx3cr1^{CreER-Eyfp/wt} mouse brains. Genetically mediated microglia depletion using Cx3cr1^{CreER-Eyfp/wt}Rosa26^DTA/wt mice and CSF1 receptor inhibitor PLX3397 were used to deplete microglia. Primary microglia proliferation and migration assay were used for in vitro studies.

Results

We unexpectedly identified a subpopulati

SUBMITTER: Zhou K 

PROVIDER: S-EPMC8783445 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature