TRF3008A suppresses the progression and metastasis of colorectal cancer by destabilizing FOXK1 in an AGO-dependent manner.

ABSTRACT:

Background

tRNA-derived fragments (tRFs) have been shown to have critical regulatory roles in cancer biology. However, the contributions of tRFs to colorectal cancer (CRC) remain largely unknown.

Methods

tRF3008A (a tRFRNA derived from tRNA^Val) was identified by RNA sequencing and validated by quantitative reverse transcription PCR. The role of tRF3008A in CRC progression was assessed both in vitro and in vivo, and its downstream target genes were identified and validated in CRC cells. RNA pull-down with mass spectrometry and AGO-RIP were used to confirm the interaction of tRF3008A and AGO proteins. The clinical implications of tRF3008A were assessed in CRC tissues and blood samples.

Results

The expression of tRF3008A was reduced in colorectal cancer, and i

SUBMITTER: Han Y 

PROVIDER: S-EPMC8783529 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature