Floxed exon (Flexon): A flexibly positioned stop cassette for recombinase-mediated conditional gene expression.

ABSTRACT: Conditional gene expression is a powerful tool for genetic analysis of biological phenomena. In the widely used "lox-stop-lox" approach, insertion of a stop cassette consisting of a series of stop codons and polyadenylation signals flanked by lox sites into the 5' untranslated region (UTR) of a gene prevents expression until the cassette is excised by tissue-specific expression of Cre recombinase. Although lox-stop-lox and similar approaches using other site-specific recombinases have been successfully used in many experimental systems, this design has certain limitations. Here, we describe the Floxed exon (Flexon) approach, which uses a stop cassette composed of an artificial exon flanked by artificial introns, designed to cause premature termination of translation and nonsense-med

SUBMITTER: Shaffer JM 

PROVIDER: S-EPMC8784106 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature