Involvement of FoxO1, Sp1, and Nrf2 in Upregulation of Negative Regulator of ROS by 15d-PGJ₂ Attenuates H₂O₂-Induced IL-6 Expression in Rat Brain Astrocytes.

ABSTRACT: Excessive production of reactive oxygen species (ROS) by NADPH oxidase (Nox) resulted in inflammation. The negative regulator of ROS (NRROS) dampens ROS generation during inflammatory responses. 15-Deoxy-∆12,14-prostaglandin J₂ (15d-PGJ₂) exhibits neuroprotective effects on central nervous system (CNS). However, whether 15d-PGJ₂-induced NRROS expression was unknown in rat brain astrocytes (RBA-1). NRROS expression was determined by Western blot, RT/real-time PCR, and promoter activity assays. The signaling components were investigated using pharmacological inhibitors or specific siRNAs. The interaction between transcription factors and the NRROS promoter was investigated by chromatin immunoprecipitation assay. Upregulation of NRROS on the hydrogen peroxide

SUBMITTER: Wang CY 

PROVIDER: S-EPMC8784370 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature