Simulated Microgravity Potentiates Hematopoietic Differentiation of Human Pluripotent Stem Cells and Supports Formation of 3D Hematopoietic Cluster.

ABSTRACT: Microgravity has been shown to induces many changes in proliferation, differentiation and growth behavior of stem cells. Little is known about the effect of microgravity on hematopoietic differentiation of pluripotent stem cells (PSCs). In this study, we used the random position machine (RPM) to investigate whether simulated microgravity (SMG) allows the induction of hematopoietic stem/progenitor cell (HSPC) derived from human embryonic stem cells (hESCs) in vitro. The results showed that SMG facilitates hESCs differentiate to HSPC with more efficient induction of CD34⁺CD31⁺ hemogenic endothelium progenitors (HEPs) on day 4 and CD34⁺CD43⁺ HSPC on day 7, and these cells shows an increased generation of functional hematopoietic cells in colo

SUBMITTER: Ma C 

PROVIDER: S-EPMC8784808 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature