Untargeted Stable Isotope Probing of the Gut Microbiota Metabolome Using ¹³C-Labeled Dietary Fibers.

ABSTRACT: The gut microbiome generates numerous metabolites that exert local effects and enter the circulation to affect the functions of many organs. Despite extensive sequencing-based characterization of the gut microbiome, there remains a lack of understanding of microbial metabolism. Here, we developed an untargeted stable isotope-resolved metabolomics (SIRM) approach for the holistic study of gut microbial metabolites. Viable microbial cells were extracted from fresh mice feces and incubated anaerobically with ¹³C-labeled dietary fibers including inulin or cellulose. High-resolution mass spectrometry was used to monitor ¹³C enrichment in metabolites associated with glycolysis, the Krebs cycle, the pentose phosphate pathway, nucleotide synthesis, and pyruvate catabolism in

SUBMITTER: Deng P 

PROVIDER: S-EPMC8784920 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature