Refining internal bilayer structure of bicelles resolved by extended-q small angle X-ray scattering.

ABSTRACT: The internal profile across the bilayer reveals important structural information regarding the crystallinity of acyl chains or the positions of encapsulated species. Here, we demonstrate that a simple five-layer-core-shell discoidal model can be employed to best fit the extended-q small angle X-ray scattering (SAXS) data and resolve the bilayer internal structure (with sub-nanometer resolution) of a nanoscale discoidal system comprised of a mixture of long- and short- chain lipids (known as "bicelles"). In contrast to the traditional core-shell discoidal model, the detailed structure in the hydrophobic core such as the methylene and methyl groups can be distinguished via this model. The refined model is validated by the SAXS data of bicelles whose electron scattering length density of the

SUBMITTER: Cheu C 

PROVIDER: S-EPMC8784952 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature