Postnatal maturation of calcium signaling in islets of Langerhans from neonatal mice.

ABSTRACT: Pancreatic islet cells develop mature physiological responses to glucose and other fuels postnatally. In this study, we used fluorescence imaging techniques to measure changes in intracellular calcium ([Ca²⁺]_i) to compare islets isolated from mice on postnatal days 0, 4, and 12 with islets from adult CD-1 mice. In addition, we used publicly available RNA-sequencing data to compare expression levels of key genes in β-cell physiology with [Ca²⁺]_i data across these ages. We show that islets isolated from mice on postnatal day 0 displayed elevated [Ca²⁺]_i in basal glucose (≤4 mM) but lower [Ca²⁺]_i responses to stimulation by 12-20 mM glucose compared to adult. Neonatal islets displayed more adult-like [Ca

SUBMITTER: West HL 

PROVIDER: S-EPMC8785357 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature