Human papillomavirus E6E7 mRNA and TERC lncRNA in situ detection in cervical scraped cells and cervical disease progression assessment.

ABSTRACT:

Background

Human papillomavirus screen in female cervical cells has demonstrated values in clinical diagnosis of precancerous lesions and cervical cancers. Human papillomavirus tests of cervical cells by utilizing Polymerase Chain Reaction (PCR) method provides human papillomavirus infection status however no further virus in situ information. Although it is well known that the tests of human papillomavirus E6/E7 RNA location in infected cervical cells and cell internal malignancy molecular will provide clues for gynecologists to evaluate disease progression, there are technique difficulties to preserve RNAs in cervical scraped cells for in situ hybridization.

Methods

In current study, after developing a cervical cell collection and preparation method for RNA in situ hybridi

SUBMITTER: Zhao H 

PROVIDER: S-EPMC8785513 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature