Open chromatin interaction maps reveal functional regulatory elements and chromatin architecture variations during wheat evolution.

ABSTRACT:

Background

Bread wheat (Triticum aestivum) is an allohexaploid that is generated by two subsequent allopolyploidization events. The large genome size (16 Gb) and polyploid complexity impede our understanding of how regulatory elements and their interactions shape chromatin structure and gene expression in wheat. The open chromatin enrichment and network Hi-C (OCEAN-C) is a powerful antibody-independent method to detect chromatin interactions between open chromatin regions throughout the genome.

Results

Here we generate open chromatin interaction maps for hexaploid wheat and its tetraploid and diploid relatives using OCEAN-C. The anchors of chromatin loops show high chromatin accessibility and are concomitant with several active histone modifications, with 67% of them interac

SUBMITTER: Yuan J 

PROVIDER: S-EPMC8785527 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature