Autoantigen microarrays reveal myelin basic protein autoantibodies in morphea.

ABSTRACT:

Background

Morphea is an autoimmune, sclerosing skin disorder. Despite the recent emphasis on immune dysregulation in morphea, the role of autoantibodies in morphea pathogenesis or utility as biomarkers are poorly defined.

Methods

Autoantigen microarray was used to profile autoantibodies from the serum of participants from the Morphea in Adults and Children (MAC) cohort. Clinical and demographic features of morphea patients with myelin basic protein (MBP) autoantibodies were compared to those without. MBP immunohistochemistry staining was subsequently performed in morphea skin to assess for perineural inflammation in areas of staining. Immunofluorescence staining on mouse brain tissue was also performed using patient sera and mouse anti-myelin basic protein antibody to confi

SUBMITTER: Zhu JL 

PROVIDER: S-EPMC8785566 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature