Interventional- and amputation-stage muscle proteomes in the chronically threatened ischemic limb.

ABSTRACT:

Background

Despite improved surgical approaches for chronic limb-threatening ischemia (CLTI), amputation rates remain high and contributing tissue-level factors remain unknown. The purpose of this study was twofold: (1) to identify differences between the healthy adult and CLTI limb muscle proteome, and (2) to identify differences in the limb muscle proteome of CLTI patients prior to surgical intervention or at the time of amputation.

Methods and results

Gastrocnemius muscle was collected from non-ischemic controls (n = 19) and either pre-interventional surgery (n = 10) or at amputation outcome (n = 29) CLTI patients. All samples were subjected to isobaric tandem-mass-tag-assisted proteomics. The mitochondrion was the primary classification of downregulated proteins (> 70%)

SUBMITTER: Ryan TE 

PROVIDER: S-EPMC8785983 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature