Peripheral mitochondrial DNA, telomere length and DNA methylation as predictors of live birth in in vitro fertilization cycles.

ABSTRACT:

Objective

To evaluate whether telomere length (TL), mitochondrial-DNA (mt-DNA) or epigenetic age estimators based on DNA methylation (DNAm) pattern could be considered reliable predictors of in-vitro-fertilization (IVF) success in terms of live birth rate.

Design

Prospective cohort study.

Setting

Infertility Unit of the Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico.

Patients

181 women aged 37-39 years who underwent IVF at a single-centre between January 2017 and December 2018.

Interventions

On the day of recruitment, blood samples were collected, and genomic DNA was isolated from white blood cells. TL, mt-DNA and DNAm assessment was performed using quantitative real-time polymerase chain reaction (qPCR). Biological age (DNAm age) was computed

SUBMITTER: Li Piani L 

PROVIDER: S-EPMC8786209 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature