Using cryo-EM to uncover mechanisms of bacterial transcriptional regulation.

ABSTRACT: Transcription is the principal control point for bacterial gene expression, and it enables a global cellular response to an intracellular or environmental trigger. Transcriptional regulation is orchestrated by transcription factors, which activate or repress transcription of target genes by modulating the activity of RNA polymerase. Dissecting the nature and precise choreography of these interactions is essential for developing a molecular understanding of transcriptional regulation. While the contribution of X-ray crystallography has been invaluable, the 'resolution revolution' of cryo-electron microscopy has transformed our structural investigations, enabling large, dynamic and often transient transcription complexes to be resolved that in many cases had resisted crystallisation. In this

SUBMITTER: Wood DM 

PROVIDER: S-EPMC8786299 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature