Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C₃ and C₄ plants.

ABSTRACT: Photosynthesis-related pathways are regarded as a promising avenue for crop improvement. Whilst empirical studies have shown that photosynthetic efficiency is higher in microalgae than in C₃ or C₄ crops, the underlying reasons remain unclear. Using a tailor-made microfluidics labelling system to supply ¹³CO₂ at steady state, we investigated in vivo labelling kinetics in intermediates of the Calvin Benson cycle and sugar, starch, organic acid and amino acid synthesis pathways, and in protein and lipids, in Chlamydomonas reinhardtii, Chlorella sorokiniana and Chlorella ohadii, which is the fastest growing green alga on record. We estimated flux patterns in these algae and compared them with published and new data from C₃ and C₄

SUBMITTER: Treves H 

PROVIDER: S-EPMC8786664 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature