Meiosis-Based Laboratory Evolution of the Thermal Tolerance in Kluyveromyces marxianus.

ABSTRACT: Kluyveromyces marxianus is the fastest-growing eukaryote and a promising host for producing bioethanol and heterologous proteins. To perform a laboratory evolution of thermal tolerance in K. marxianus, diploid, triploid and tetraploid strains were constructed, respectively. Considering the genetic diversity caused by genetic recombination in meiosis, we established an iterative cycle of "diploid/polyploid - meiosis - selection of spores at high temperature" to screen thermotolerant strains. Results showed that the evolution of thermal tolerance in diploid strain was more efficient than that in triploid and tetraploid strains. The thermal tolerance of the progenies of diploid and triploid strains after a two-round screen was significantly improved than that after a one-

SUBMITTER: Wu L 

PROVIDER: S-EPMC8786734 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature