VDJ Gene Usage in IgM Repertoires of Rhesus and Cynomolgus Macaques.

ABSTRACT: Macaques are frequently used to evaluate candidate vaccines and to study infection-induced antibody responses, requiring an improved understanding of their naïve immunoglobulin (IG) repertoires. Baseline gene usage frequencies contextualize studies of antigen-specific immune responses, providing information about how easily one may stimulate a response with a particular VDJ recombination. Studies of human IgM repertoires have shown that IG VDJ gene frequencies vary several orders of magnitude between the most and least utilized genes in a manner that is consistent across many individuals but to date similar analyses are lacking for macaque IgM repertoires. Here, we quantified VDJ gene usage levels in unmutated IgM repertoires of 45 macaques, belonging to two species and four commonly used

SUBMITTER: Chernyshev M 

PROVIDER: S-EPMC8786739 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature