A large scale mass spectrometry-based histone screening for assessing epigenetic developmental toxicity.

ABSTRACT: Toxicoepigenetics is an emerging field that studies the toxicological impact of compounds on protein expression through heritable, non-genetic mechanisms, such as histone post-translational modifications (hPTMs). Due to substantial progress in the large-scale study of hPTMs, integration into the field of toxicology is promising and offers the opportunity to gain novel insights into toxicological phenomena. Moreover, there is a growing demand for high-throughput human-based in vitro assays for toxicity testing, especially for developmental toxicity. Consequently, we developed a mass spectrometry-based proof-of-concept to assess a histone code screening assay capable of simultaneously detecting multiple hPTM-changes in human embryonic stem cells. We first validated the untargeted workflow wi

SUBMITTER: Verhelst S 

PROVIDER: S-EPMC8786925 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature