Quantum optical immunoassay: upconversion nanoparticle-based neutralizing assay for COVID-19.

ABSTRACT: In a viral pandemic, a few important tests are required for successful containment of the virus and reduction in severity of the infection. Among those tests, a test for the neutralizing ability of an antibody is crucial for assessment of population immunity gained through vaccination, and to test therapeutic value of antibodies made to counter the infections. Here, we report a sensitive technique to detect the relative neutralizing strength of various antibodies against the SARS-CoV-2 virus. We used bright, photostable, background-free, fluorescent upconversion nanoparticles conjugated with SARS-CoV-2 receptor binding domain as a phantom virion. A glass bottom plate coated with angiotensin-converting enzyme 2 (ACE-2) protein imitates the target cells. When no neutralizing IgG antibody was

SUBMITTER: Rajil N 

PROVIDER: S-EPMC8786937 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature