Metformin promotes histone deacetylation of optineurin and suppresses tumour growth through autophagy inhibition in ocular melanoma.

ABSTRACT:

Objective

To explore the therapeutic potential and the underlying mechanism of metformin, an adenosine monophosphate-activated kinase (AMPK) activator, in ocular melanoma.

Methods

CCK8, transwell, and colony formation assays were performed to detect the proliferation and migration ability of ocular melanoma cells. A mouse orthotopic xenograft model was built to detect ocular tumor growth in vivo. Western blot, immunofluorescence, and electron microscopy were adopted to evaluate the autophagy levels of ocular melanoma cells, and high-throughput proteomics and CUT & Tag assays were performed to analyze the candidate for autophagy alteration.

Results

Here, we revealed for the first time that a relatively low dose of metformin induced significant tumorspecific inhibition

SUBMITTER: Zhuang A 

PROVIDER: S-EPMC8787022 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature