Intracellular Thermal Probing Using Aggregated Fluorescent Nanodiamonds.

ABSTRACT: Intracellular thermometry provides important information about the physiological activity of single cells and has been implemented using diverse temperature-sensitive materials as nanoprobes. However, measuring the temperature of specific organelles or subcellular structures is challenging because it requires precise positioning of the nanoprobes. Here, it is shown that dispersed fluorescent nanodiamonds (FNDs) endocytosed in living cells can be aggregated into microspheres using optical forces and used as intracellular temperature probes. The aggregation of the FNDs and electromagnetic resonance between individual nanodiamonds in the microspheres lead to a sevenfold intensity enhancement of 546-nm laser excitation. With the assistance of a scanning optical tweezing system, the FND microsp

SUBMITTER: Wu T 

PROVIDER: S-EPMC8787390 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature