Comprehensive Analysis of Differentially Expressed Profiles of mRNA N6-Methyladenosine in Colorectal Cancer.

ABSTRACT: Aim: To comprehensively profile the landscape of the mRNA N⁶-methyladenosine (m⁶A) modification in human colorectal cancer (CRC). Methods: Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was explored to compare the difference in mRNA N⁶-methyladenosine (m⁶A) methylation between CRC tissues and adjacent normal control (NC) tissue. RNA-sequencing (RNA-seq) was performed to transcribe differentially expressed mRNAs. Conjoint analysis of MeRIP-seq and RNA-seq data was conducted to predict RNA-binding proteins (RBPs). Results: MeRIP-seq identified 1110 differentially m⁶A methylated sites (DMMSs) and 980 differentially m⁶A methylated genes (DMMGs) in CRC, with 50.13% of all modified genes showing uni

SUBMITTER: Li N 

PROVIDER: S-EPMC8787460 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature