Distinguishing Histidine Tautomers in Proteins Using Covalent Labeling-Mass Spectrometry.

ABSTRACT: In this work, we use diethylpyrocarbonate (DEPC)-based covalent labeling together with LC-MS/MS analysis to distinguish the two sidechain tautomers of histidine residues in peptides and proteins. From labeling experiments on model peptides, we demonstrate that DEPC reacts equally with both tautomeric forms to produce chemically different products with distinct dissociation patterns and LC retention times, allowing the ratios of the two tautomers to be determined in peptides and proteins. Upon measuring the tautomer ratios of several histidine residues in myoglobin, we find good agreement with previous 2D NMR data on this protein. Because our DEPC labeling/MS approach is simpler, faster, and more precise than 2D NMR, our method will be a valuable way to determine how protein structure enfor

SUBMITTER: Pan X 

PROVIDER: S-EPMC8787799 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature