Cell-Free Gene Expression Dynamics in Synthetic Cell Populations.

ABSTRACT: The ability to build synthetic cellular populations from the bottom-up provides the groundwork to realize minimal living tissues comprising single cells which can communicate and bridge scales into multicellular systems. Engineered systems made of synthetic micron-sized compartments and integrated reaction networks coupled with mathematical modeling can facilitate the design and construction of complex and multiscale chemical systems from the bottom-up. Toward this goal, we generated populations of monodisperse liposomes encapsulating cell-free expression systems (CFESs) using double-emulsion microfluidics and quantified transcription and translation dynamics within individual synthetic cells of the population using a fluorescent Broccoli RNA aptamer and mCherry protein reporter. CFE dynam

SUBMITTER: Gonzales DT 

PROVIDER: S-EPMC8787815 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature