A novel prognostic model based on single-cell RNA sequencing data for hepatocellular carcinoma.

ABSTRACT:

Background

The tumour heterogeneous make-up of immune cell infiltrates is a key factor for the therapy response and prognosis of hepatocellular carcinoma (HCC). However, it is still a major challenge to comprehensively understand the tumour immune microenvironment (TIME) at the genetic and cellular levels.

Methods

HCC single-cell RNA sequencing (scRNA-seq) data were downloaded from the Gene Expression Omnibus (GEO) database, and gene expression data were retrieved from The Cancer Genome Atlas (TCGA) database and International Cancer Genome Consortium (ICGC) database. Cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) was performed to evaluate the abundance of immune infiltrating cells. We employed weighted gene coexpression network analysi

SUBMITTER: Lu J 

PROVIDER: S-EPMC8787928 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature