The essential roles of m⁶A RNA modification to stimulate ENO1-dependent glycolysis and tumorigenesis in lung adenocarcinoma.

ABSTRACT:

Background

Lung adenocarcinoma (LUAD)  is the most common subtype of lung cancer. Patient prognosis is poor, and the existing therapeutic strategies for LUAD are far from satisfactory. Recently, targeting N6-methyladenosine (m⁶A) modification of RNA has been suggested as a potential strategy to impede tumor progression. However, the roles of m⁶A modification in LUAD tumorigenesis is unknown.

Methods

Global m⁶A levels and expressions of m⁶A writers, erasers and readers were evaluated by RNA methylation assay, dot blot, immunoblotting, immunohistochemistry and ELISA in human LUAD, mouse models and cell lines. Cell viability, 3D-spheroid generation, in vivo LUAD formation, experiments in cell- and patient-derived xenograft mice and su

SUBMITTER: Ma L 

PROVIDER: S-EPMC8788079 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature