Conformational Dynamics of Histone H3 Tails in Chromatin.

ABSTRACT: Chromatin is a supramolecular DNA-protein complex that compacts eukaryotic genomes and regulates their accessibility and functions. Dynamically disordered histone H3 N-terminal tails are among key chromatin regulatory components. Here, we used high-resolution-magic-angle-spinning NMR measurements of backbone amide ¹⁵N spin relaxation rates to investigate, with residue-specific detail, the dynamics and interactions of H3 tails in recombinant ¹³C,¹⁵N-enriched nucleosome arrays containing 15, 30, or 60 bp linker DNA between the nucleosome repeats. These measurements were compared to analogous data available for mononucleosomes devoid of linker DNA or containing two 20 bp DNA overhangs. The H3 tail dynamics in nucleosome arrays were found to be considerably att

SUBMITTER: Zandian M 

PROVIDER: S-EPMC8788308 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature