Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance.

ABSTRACT: In proteomics, it is essential to quantify proteins in absolute terms if we wish to compare results among studies and integrate high-throughput biological data into genome-scale metabolic models. While labeling target peptides with stable isotopes allow protein abundance to be accurately quantified, the utility of this technique is constrained by the low number of quantifiable proteins that it yields. Recently, label-free shotgun proteomics has become the "gold standard" for carrying out global assessments of biological samples containing thousands of proteins. However, this tool must be further improved if we wish to accurately quantify absolute levels of proteins. Here, we used different label-free quantification techniques to estimate absolute protein abundance in the model yeast Saccha

SUBMITTER: Millan-Oropeza A 

PROVIDER: S-EPMC8788469 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature