LncRNAs Are Differentially Expressed between Wildtype and Cell Line Strains of African Trypanosomes.

ABSTRACT: Trypanosoma brucei is a parasitic protist that causes African sleeping sickness. The establishment of T. brucei cell lines has provided a significant advantage for the majority of T. brucei research. However, these cell lines were isolated and maintained in culture for decades, occasionally accumulating changes in gene expression. Since trypanosome strains have been maintained in culture for decades, it is possible that difference may have accumulated in fast-evolving non-coding RNAs between trypanosomes from the wild and those maintained extensively in cultures. To address this, we compared the lncRNA expression profile of trypanosomes maintained as cultured cell lines (CL) to those extracted from human patients, wildtype (WT). We identified lncRNAs from CL and WT from available transcrip

SUBMITTER: Kim HC 

PROVIDER: S-EPMC8788480 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature