Detecting Flavobacterial Fish Pathogens in the Environment via High-Throughput Community Analysis.

ABSTRACT: Diseases caused by the fish pathogens Flavobacterium columnare and Flavobacterium psychrophilum are major contributors of preventable losses in the aquaculture industry. The persistent and difficult-to-control infections caused by these bacteria make timely intervention and prophylactic elimination of pathogen reservoirs important measures to combat these disease-causing agents. In this study, we present two independent assays for detecting these pathogens in a range of environmental samples. Natural water samples were inoculated with F. columnare and F. psychrophilum over 5 orders of magnitude, and pathogen levels were detected using Illumina MiSeq sequencing and droplet digital PCR. Both detection methods accurately identified pathogen-positive samples and showed good agreement in quanti

SUBMITTER: Testerman T 

PROVIDER: S-EPMC8788675 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature