Viewing Cortical Collecting Duct Function Through Phenotype-guided Single-Tubule Proteomics.

ABSTRACT: The revolution of the omics technologies has enabled profiling of the molecules of any sample. However, the heterogeneity of the kidney with highly specialized nephron segments like the cortical collecting duct (CCD) poses a challenge regarding integration of omics data and functional analysis. We examined function and proteome from the same single CCDs of C57Bl6 mice by investigating them in a double-barreled perfusion system before targeted mass spectrometry. Transepithelial voltage (V_te), transepithelial resistance, as well as amiloride-sensitive voltage (ΔV_teamil) were recorded. CCDs were of 400-600 µm of length, showed lumen negative V_te between -8.5 and -32.5 mV and an equivalent short circuit current I'_sc between 54 and 192 µA/cm²

SUBMITTER: Himmerkus N 

PROVIDER: S-EPMC8788781 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature