Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling.

ABSTRACT: The epidermis forms an essential barrier against a variety of insults. The overall goal of this study was to shed light not only on the effects of accidental epidermal injury, but also on the mechanisms that support laser skin resurfacing with intra-epidermal focal laser-induced photodamage, a widespread medical practice used to treat a range of skin conditions. To this end, we selectively photodamaged a single keratinocyte with intense, focused and pulsed laser radiation, triggering Ca²⁺ waves in the epidermis of live anesthetized mice with ubiquitous expression of a genetically encoded Ca²⁺ indicator. Waves expanded radially and rapidly, reaching up to eight orders of bystander cells that remained activated for tens of minutes, without displaying oscillations of the

SUBMITTER: Donati V 

PROVIDER: S-EPMC8788836 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature