A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals.

ABSTRACT: Mitochondria play a key role in cellular calcium (Ca²⁺) homeostasis. Dysfunction in the organelle Ca²⁺ handling appears to be involved in several pathological conditions, ranging from neurodegenerative diseases, cardiac failure and malignant transformation. In the past years, several targeted green fluorescent protein (GFP)-based genetically encoded Ca²⁺ indicators (GECIs) have been developed to study Ca²⁺ dynamics inside mitochondria of living cells. Surprisingly, while there is a number of transgenic mice expressing different types of cytosolic GECIs, few examples are available expressing mitochondria-localized GECIs, and none of them exhibits adequate spatial resolution. Here we report the generation and characterization of a transgenic mouse

SUBMITTER: Redolfi N 

PROVIDER: S-EPMC8788866 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature