Analysis of HubP-dependent cell pole protein targeting in Vibrio cholerae uncovers novel motility regulators.

ABSTRACT: In rod-shaped bacteria, the emergence and maintenance of long-axis cell polarity is involved in key cellular processes such as cell cycle, division, environmental sensing and flagellar motility among others. Many bacteria achieve cell pole differentiation through the use of polar landmark proteins acting as scaffolds for the recruitment of functional macromolecular assemblies. In Vibrio cholerae a large membrane-tethered protein, HubP, specifically interacts with proteins involved in chromosome segregation, chemotaxis and flagellar biosynthesis. Here we used comparative proteomics, genetic and imaging approaches to identify additional HubP partners and demonstrate that at least six more proteins are subject to HubP-dependent polar localization. These include a cell-wall remodeling enzyme (

SUBMITTER: Altinoglu I 

PROVIDER: S-EPMC8789113 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature