Natural compound library screening identifies Sanguinarine chloride for the treatment of SCLC by upregulating CDKN1A.

ABSTRACT:

Objectives

Small cell lung cancer (SCLC) is notorious for aggressive malignancy without effective treatment, and most patients eventually develop tumor progression with a poor prognosis. There is an urgent need for discovering novel antitumor agents or therapeutic strategies for SCLC.

Materials and methods

We performed a screening method based on CCK-8 assay to screen 640 natural compounds for SCLC. The effects of Sanguinarine chloride on SCLC cell proliferation, colony formation, cell cycle, apoptosis, migration and invasion were determined. RNA-seq and bioinformatics analysis was performed to investigate the anti-SCLC mechanism of Sanguinarine chloride. Publicly available datasets and samples were analyzed to investigate the expression level of CDKN1A and its clinical sign

SUBMITTER: Zhong M 

PROVIDER: S-EPMC8789530 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature