Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.

ABSTRACT: Viral pathogens are an ongoing threat to public health worldwide. Analysing their dependence on host biosynthetic pathways could lead to effective antiviral therapies¹. Here we integrate proteomic analyses of polysomes with functional genomics and pharmacological interventions to define how enteroviruses and flaviviruses remodel host polysomes to synthesize viral proteins and disable host protein production. We find that infection with polio, dengue or Zika virus markedly modifies polysome composition, without major changes to core ribosome stoichiometry. These viruses use different strategies to evict a common set of translation initiation and RNA surveillance factors from polysomes while recruiting host machineries that are specifically required for viral biogenesis. Targeting

SUBMITTER: Aviner R 

PROVIDER: S-EPMC8789550 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature