The effects of ARID1A mutations on colorectal cancer and associations with PD-L1 expression by stromal cells.

ABSTRACT:

Background

ARID1A is a component of the SWI/SNF complex, which controls the accessibility of proteins to DNA. ARID1A mutations are frequently observed in colorectal cancers (CRCs) and have been reported to be associated with high mutational burden and tumor PD-L1 expression in vitro.

Aim

To clarify the role of ARID1A mutation in CRC.

Method and results

We used next generation sequencing (NGS) and immunohistochemistry on clinically obtained samples. A total of 201 CRC tissues from Niigata University and Niigata Center Hospital were processed by NGS using the CANCERPLEX panel. Immunohistochemistry for ARID1A, PD-L1, MLH1, and MSH2 was performed on 66 propensity-matched (33 microsatellite instability-high [MSI-H] and 33 microsatellite-stable [MSS]) cases among 499 cases

SUBMITTER: Kamori T 

PROVIDER: S-EPMC8789618 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature