Effect of delayed entry of blood culture bottles in BACTEC automated blood culture system in the context of laboratory consolidation.

ABSTRACT: Delayed entry of blood culture bottles is frequent in consolidated laboratories. A retrospective study evaluated time from insertion to detection and total detection time as a function of preincubation time, and we prospectively looked for false negative results. 69,604 blood culture bottles were reviewed for preincubation time, incubation time and total detection time. Positive cultures for specific bacterial subtypes were reviewed to assess the effect of preincubation time on likelihood of detection. 492 negative blood cultures were prospectively tested by 16S RNA PCR and Staphylococcus-specific PCR for the presence of bacterial DNA. Mean preincubation time for samples collected within the city-limits was 3.94 h versus 9.49-18.89 h for other client sites. Higher preincubation times were

SUBMITTER: Deslandes V 

PROVIDER: S-EPMC8789921 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature