Complexome Profiling-Exploring Mitochondrial Protein Complexes in Health and Disease.

ABSTRACT: Complexome profiling (CP) is a state-of-the-art approach that combines separation of native proteins by electrophoresis, size exclusion chromatography or density gradient centrifugation with tandem mass spectrometry identification and quantification. Resulting data are computationally clustered to visualize the inventory, abundance and arrangement of multiprotein complexes in a biological sample. Since its formal introduction a decade ago, this method has been mostly applied to explore not only the composition and abundance of mitochondrial oxidative phosphorylation (OXPHOS) complexes in several species but also to identify novel protein interactors involved in their assembly, maintenance and functions. Besides, complexome profiling has been utilized to study the dynamics of OXPHOS complex

SUBMITTER: Cabrera-Orefice A 

PROVIDER: S-EPMC8790184 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature