Isothermal titration calorimetry of membrane protein interactions: FNR and the cytochrome b₆f complex.

ABSTRACT: Ferredoxin-NADP⁺ reductase (FNR) was previously inferred to bind to the cytochrome b₆f complex in the electron transport chain of oxygenic photosynthesis. In the present study, this inference has been examined through analysis of the thermodynamics of the interaction between FNR and the b₆f complex. Isothermal titration calorimetry (ITC) was used to characterize the physical interaction of FNR with b₆f complex derived from two plant sources (Spinacia oleracea and Zea maize). ITC did not detect a significant interaction of FNR with the b₆f complex in detergent solution nor with the complex reconstituted in liposomes. A previous inference of a small amplitude but defined FNR-b₆f interaction is explained by FNR interaction with

SUBMITTER: Zakharov SD 

PROVIDER: S-EPMC8790201 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature