The molecular role of Sigmar1 in regulating mitochondrial function through mitochondrial localization in cardiomyocytes.

ABSTRACT: Sigmar1 is a widely expressed molecular chaperone protein in mammalian cell systems. Accumulating research demonstrated the cardioprotective roles of pharmacologic Sigmar1 activation by ligands in preclinical rodent models of cardiac injury. Extensive biochemical and immuno-electron microscopic research demonstrated Sigmar1's sub-cellular localization largely depends on cell and organ types. Despite comprehensive studies, Sigmar1's direct molecular role in cardiomyocytes remains elusive. In the present study, we determined Sigmar1's subcellular localization, transmembrane topology, and function using complementary microscopy, biochemical, and functional assays in cardiomyocytes. Quantum dots in transmission electron microscopy showed Sigmar1 labeled quantum dots on the mitochondrial membra

SUBMITTER: Abdullah CS 

PROVIDER: S-EPMC8790786 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature