New Insights of Transcriptional Regulator AflR in Aspergillus flavus Physiology.

ABSTRACT: Aspergillus flavus aflR, a gene encoding a Zn(II)₂Cys₆ DNA-binding domain, is an important transcriptional regulator of the aflatoxin biosynthesis gene cluster. Our previous results of Gene ontology (GO) analysis for the binding sites of AflR in A. flavus suggest that AflR may play an integrative regulatory role. In this study the ΔaflR and overexpression (OE) strains based on the well-established double-crossover recombinational technique were constructed to investigate the integrative function of the aflR gene in A. flavus. The disruption of aflR severely affected the aflatoxin biosynthetic pathway, resulting in a significant decrease in aflatoxin production. The aflatoxin B₁ (AFB₁) of the ΔaflR strain was 180

SUBMITTER: Wang P 

PROVIDER: S-EPMC8791188 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature