Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery.

ABSTRACT: Most mitochondrial proteins are translated in the cytosol and imported into mitochondria. Mutations in the mitochondrial protein import machinery cause human pathologies. However, a lack of suitable tools to measure protein uptake across the mitochondrial proteome has prevented the identification of specific proteins affected by import perturbation. Here, we introduce mePROD^mt, a pulsed-SILAC based proteomics approach that includes a booster signal to increase the sensitivity for mitochondrial proteins selectively, enabling global dynamic analysis of endogenous mitochondrial protein uptake in cells. We applied mePROD^mt to determine protein uptake kinetics and examined how inhibitors of mitochondrial import machineries affect protein uptake. Monitoring changes in trans

SUBMITTER: Schafer JA 

PROVIDER: S-EPMC8791276 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature