Twist1 in T Lymphocytes Augments Kidney Fibrosis after Ureteral Obstruction.

ABSTRACT:

Background

Twist1 is a basic helix-loop-helix domain-containing transcription factor that participates in diverse cellular functions, including epithelial-mesenchymal transition and the cellular immune response. Although Twist1 plays critical roles in the initiation and progression of kidney diseases, the effects of Twist1 in the T lymphocyte on the progression of renal fibrosis require elucidation.

Methods

129/SvEv mice with a floxed allele for the gene encoding Twist1 or TNFα were bred with CD4-Cre mice to yield CD4-Cre⁺ Twist1^flox/flox (Twist1-TKO) or CD4-Cre⁺ TNF^flox/flox (TNF-TKO) mice with robust, but selective, deletion of Twist1 or TNFα mRNA in T cells, respectively. Twist1 TKO, TNF TKO, and WT controls underw

SUBMITTER: Ren J 

PROVIDER: S-EPMC8791343 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature