Human blastoids model blastocyst development and implantation.

ABSTRACT: One week after fertilization, human embryos implant into the uterus. This event requires the embryo to form a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model that we called a blastoid¹. Here we show that naive human pluripotent stem cells cultured in PXGL medium² and triply inhibited for the Hippo, TGF-β and ERK pathways efficiently (with more than 70% efficiency) form blastoids generating blastocyst-stage analogues of the three founding lineages (more than 97% trophectoderm, epiblast and primitive endoderm) according to the sequence and timing of blastocyst development. Blastoids spontaneously form the first axis, and we observe that the epiblast induces the local maturation of the polar trophect

SUBMITTER: Kagawa H 

PROVIDER: S-EPMC8791832 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature