Metagenomic sequencing for detection and identification of the boxwood blight pathogen Calonectria pseudonaviculata.

ABSTRACT: Pathogen detection and identification are key elements in outbreak control of human, animal, and plant diseases. Since many fungal plant pathogens cause similar symptoms, are difficult to distinguish morphologically, and grow slowly in culture, culture-independent, sequence-based diagnostic methods are desirable. Whole genome metagenomic sequencing has emerged as a promising technique because it can potentially detect any pathogen without culturing and without the need for pathogen-specific probes. However, efficient DNA extraction protocols, computational tools, and sequence databases are required. Here we applied metagenomic sequencing with the Oxford Nanopore Technologies MinION to the detection of the fungus Calonectria pseudonaviculata, the causal agent of boxwood (Buxus spp.) blight

SUBMITTER: Yang S 

PROVIDER: S-EPMC8791934 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature